Bacillus megaterium
Jual Culture Bacillus megaterium
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Bacillus
megaterium has been industrially employed for more than 50 years, as it
possesses some very useful and unusual enzymes and a high capacity for the
production of exoenzymes. It is also a desirable cloning host for the
production of intact proteins, as it does not possess external alkaline
proteases and can stably maintain a variety of plasmid vectors. Genetic tools
for this species include transducing phages and several hundred mutants
covering the processes of biosynthesis, catabolism, division, sporulation,
germination, antibiotic resistance, and recombination. The seven plasmids of B.
megaterium strain QM B1551 contain several unusual metabolic genes that may be
useful in bioremediation. Recently, several recombinant shuttle vectors
carrying different strong inducible promoters and various combinations of
affinity tags for simple protein purification have been constructed. Leader
sequences-mediated export of affinity-tagged proteins into the growth medium
was made possible.
These plasmids
are commercially available. For a broader application of B. megaterium in
industry, sporulation and protease-deficient as well as UV-sensitive mutants
were constructed. The genome sequence of two different strains, plasmidless
DSM319 and QM B1551 carrying seven natural plasmids, is now available. These
sequences allow for a systems biotechnology optimization of the production host
B. megaterium. Altogether, a "toolbox" of hundreds of genetically
characterized strains, genetic methods, vectors, hosts, and genomic sequences
make B. megaterium an ideal organism for industrial, environmental, and
experimental applications.
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